Journal: Nature Metabolism

Article Title: Feeding-regulated glycogen metabolism drives rhythmic liver protein secretion

doi: 10.1038/s42255-026-01453-8

Figure Lengend Snippet: a , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in vivo. WT mice under a regular 12 h light–dark cycle and NR feeding were subcutaneously injected with either vehicle (Veh) or CP-91149 at ZT0 for tissue collections at ZT3, ZT6, ZT9, ZT12 and ZT24. b , Average food consumption of WT mice ( n = 4 biological replicates) 24 h after CP-91149 injection. c , Temporal profiles of glycogen in the liver of Veh-injected and CP-91149-injected mice ( n = 20 (5 timepoints × 4 biological replicates)). d , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated mouse liver were determined by lectin blot analysis with concanavalin A (ConA, n = 20 (5 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (right). e , C3 levels in mouse serum as assessed by ELISA ( n = 20 (5 timepoints × 4 biological replicates)). f , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in AML12 mouse hepatocytes. Treatment with Veh or CP-91149 (67 µM) was performed for 3, 6, 12 and 24 h. g , h , Kinetic profiles of UDP-glucose + UDP-galactose levels ( g ), cytidine 5′-monophospho-N-acetyl neuraminic acid (CMP-Neu5Ac) ( h , left) and uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) ( h , right) in AML12 cells upon CP-91149 treatment ( n = 16 (4 timepoints × 4 biological replicates)). i , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated AML12 cells were determined by lectin blot analysis with ConA ( n = 16 (4 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (bottom). j , ALB, FN1 and C3 levels in cell medium determined by ELISA ( n = 22–24 (4 timepoints × 6 biological replicates, with ALB 14 h Veh and 24 h CP-91149 n = 5)). k , Experimental design (created with BioRender.com ). AML12 cells were treated with Veh or CP-91149 for 14 h in the absence or presence of supplemental UDP-glucose (UDPG; 2 mM). l , m , ALB and C3 levels in cell medium ( l ) and lysates ( m ) as determined by ELISA ( n = 6 biological replicates, except for ALB under CP-91149 treatment ( n = 5)). Data are displayed as means; error bars, s.e.m. Boxplots show the median (centre line), interquartile range (box) and minimum to maximum values (whiskers). A detailed description of the statistical analysis is available in Source Data Fig. . See also Extended Data Fig. .

Article Snippet: Primary antibodies were used at the following dilutions: 1:1,000 for ATF4 (Cell Signaling Technologies, 11815), ARFGAP1 (Cell Signaling Technologies, 14608), Phospho-RPS6 (Cell Signaling Technologies, 2211), Total-RPS6 (Cell Signaling Technologies, 2217), GABARAPL1 (Genetex, GTX132664) and ConA Lectin (Vector Laboratories, B-1005) and 1:2,000 for STX4 (ProteinTech, 14988-1-AP).

Techniques: Inhibition, In Vivo, Injection, Glycoproteomics, Staining, Control, Enzyme-linked Immunosorbent Assay

Journal: Journal of Extracellular Vesicles

Article Title: Proteomic Analysis of Extracellular Vesicles Identifies CDCP1 as Critical Metastasis‐Related Glycoprotein in Lung Cancer

doi: 10.1002/jev2.70128

Figure Lengend Snippet: EVs glycoprotein enrichment and proteomic analysis. (A) Workflow for proteome identification of EV total proteins and glycoproteins. (B) Principal component analysis of 95C and 95D EVs total proteins (T‐p) and glycoproteins (G‐p). (C) Venn diagram of EVs total proteins and glycoproteins. (D) Pearson correlation coefficient matrix plot of multiple groups. The correlation coefficient was represented by a color scheme from white (negative correlation) to blue (positive correlation). (E) The volcano plot represents the quantitative analysis of N‐glycoproteins in 95D EVs versus 95C EVs. Orange represents proteins that were at least 2‐fold upregulated ( p < 0.05); blue represents proteins that were at least 2‐fold downregulated ( p < 0.05). (F) Distribution plot of the number of N‐glycosylation sites of 95C and 95D EVs. (G‐I) Comparison of the ranking, relative quantity, and unique peptides of three typical glycoproteins before and after ConA enrichment. GO analysis (J) and KEGG pathway enrichment (K) of differentially expressed glycoproteins in EVs. BP, biological process; CC, cellular component; MF, molecular function.

Article Snippet: Briefly, for 1 mg EVs protein, 1 mL of agarose bound lectin ConA (Vector Laboratories, AL‐1003) was packed into a 2 mL disposable screw end‐cap spin column with filter (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Glycoproteomics, Comparison

Journal: ACS Omega

Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

doi: 10.1021/acsomega.5c00867

Figure Lengend Snippet: Lectin detection profiles. A total of 12 plasmon shift values, measured after 15 min of immobilization, were recorded for each lectin, with the average and standard deviation values represented by each band in the graph. Each lectin is used to create the LPS profiles for each bacterial species injected at 100 μg/mL, as well as a PBS buffer blank reference. The lectins chosen are Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA). These lectins will be referred by their abbreviation for the next figures to avoid unnecessary length in the legend.

Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

Techniques: Standard Deviation, Injection

Journal: ACS Omega

Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

doi: 10.1021/acsomega.5c00867

Figure Lengend Snippet: Feature importance coefficient. Each bar illustrates the mean importance coefficients for the 7 features associated with 7 lectins, calculated from 10 randomly selected train/test splits of the data set using a Random Forest model with 100 trees.

Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

Techniques:

Journal: ACS Omega

Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

doi: 10.1021/acsomega.5c00867

Figure Lengend Snippet: Feature reduction for RF and SVM machine learning models. The best-performing lectin set from the combination of increasing number of lectins (1 to 7, from left to right) is used in a 10 repeated 3-fold cross-validation to determine the accuracy for each model. Significant differences are assessed with a t -test ( p > 0.05). An asterisk indicates a distribution with a statistically significant difference in performance compared to a distribution without it.

Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

Techniques:

Journal: ACS Omega

Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

doi: 10.1021/acsomega.5c00867

Figure Lengend Snippet: Classification Report for Different Profile Lengths with the SVM Model

Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

Techniques:

Journal: ACS Omega

Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

doi: 10.1021/acsomega.5c00867

Figure Lengend Snippet: Classification Report for Different Profile Lengths with the RF Model

Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

Techniques: